Description: Rabbit polyclonal antibody to NMDAR1 (Phospho-S890) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S890 of human NMDAR1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: GRIN1 Alternative Names: NMDAR1; Glutamate receptor ionotropic NMDA 1; GluN1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1Entrez Gene (Human): 2902Entrez Gene (Mouse) : 14810SwissProt (Human): Q05586SwissProt (Mouse) : P35438Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NMDAR1 (Phospho-S890) expression in A549 (A), U87MG (B) whole cell lysates.
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  Immunohistochemical analysis of NMDAR1 (Phospho-S890) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of NMDAR1 (Phospho-S890) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.