Description: Rabbit polyclonal antibody to BMP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human BMP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: BMP1 Alternative Names: PCOLC; Bone morphogenetic protein 1; BMP-1; Mammalian tolloid protein; mTld; Procollagen C-proteinase; PCPEntrez Gene (Human): 649Entrez Gene (Mouse) : 12153SwissProt (Human): P13497SwissProt (Mouse) : P98063Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BMP1 expression in H9C2 (A), MEF (B), HEK293T (C), A549 (D) whole cell lysates.
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Immunohistochemical analysis of BMP1 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.