Description: Rabbit polyclonal antibody to CD11c Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD11c. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ITGAX Alternative Names: CD11C; Integrin alpha-X; CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p15095 alpha chain; Leukocyte adhesion receptor p15095; CD11cEntrez Gene (Human): 3687SwissProt (Human): P20702Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD11c expression in U87MG (A), A549 (B), Myla2059 (C) whole cell lysates.
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Immunohistochemical analysis of CD11c staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.