Description: Rabbit polyclonal antibody to IFN beta Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IFN beta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: IFNB1 Alternative Names: IFB; IFNB; Interferon beta; IFN-beta; Fibroblast interferonEntrez Gene (Human): 3456Entrez Gene (Mouse) : 15977Entrez Gene (Rat) : 24481SwissProt (Human): P01574SwissProt (Mouse) : P01575SwissProt (Rat) : P70499Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IFN beta expression in Hela (A), H1688 (B), H446 (C), mouse lung (D), mouse kidney (E), rat spleen (F) whole cell lysates.
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Immunohistochemical analysis of IFN beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.