Description: Rabbit polyclonal antibody to NCOA3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NCOA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: NCOA3 Alternative Names: AIB1; BHLHE42; RAC3; TRAM1; Nuclear receptor coactivator 3; NCoA-3; ACTR; Amplified in breast cancer 1 protein; AIB-1; CBP-interacting protein; pCIP; Class E basic helix-loop-helix protein 42; bHLHe42; Receptor-associated coactivator 3; RAC-3; Steroid receptor coactivator protein 3; SRC-3; Thyroid hormone receptor activator molecule 1; TRAM-1Entrez Gene (Human): 8202SwissProt (Human): Q9Y6Q9SwissProt (Mouse) : O09000Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NCOA3 expression in AML12 (A), A2780 (B), MCF7 (C), LO2 (D) whole cell lysates.
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  Immunohistochemical analysis of NCOA3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.