Description: Rabbit polyclonal antibody to NMBR Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NMBR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: NMBR Alternative Names: Neuromedin-B receptor; NMB-R; Epididymis tissue protein Li 185a; Neuromedin-B-preferring bombesin receptorEntrez Gene (Human): 4829Entrez Gene (Mouse) : 18101Entrez Gene (Rat) : 25264SwissProt (Human): P28336SwissProt (Mouse) : O54799SwissProt (Rat) : P24053Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NMBR expression in EC9706 (A), Hela (B), PC3 (C) whole cell lysates.
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Immunofluorescent analysis of NMBR staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.