Description: Rabbit polyclonal antibody to GPR109A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR109A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: HCAR2 Alternative Names: HCAR2; GPR109A; HCA2; HM74A; NIACR1; Hydroxycarboxylic acid receptor 2; G-protein coupled receptor 109A; G-protein coupled receptor HM74A; Niacin receptor 1; Nicotinic acid receptorEntrez Gene (Human): 8843; 338442Entrez Gene (Mouse) : 80885Entrez Gene (Rat) : 353250SwissProt (Human): Q8TDS4SwissProt (Mouse) : Q9EP66SwissProt (Rat) : Q80Z39Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GPR109A expression in Myla2059 (A), MCF7 (B), mouse spleen (C), rat spleen (D) whole cell lysates.
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  Immunohistochemical analysis of GPR109A staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of GPR109A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.