Description: Rabbit polyclonal antibody to Frizzled 5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Frizzled 5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: FZD5; FZD8 Alternative Names: FZD5; C2orf31; Frizzled-5; Fz-5; hFz5; FzE5; FZD8; Frizzled-8; Fz-8; hFz8Entrez Gene (Human): 8325; 7855Entrez Gene (Mouse) : 14367; 14370Entrez Gene (Rat) : 317674; 100909849SwissProt (Human): Q13467; Q9H461SwissProt (Mouse) : Q9EQD0; Q61091SwissProt (Rat) : Q8CHL0; Q498S8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Frizzled 5 expression in SGC7901 (A), PC3 (B), MEF (C) whole cell lysates.
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  Immunofluorescent analysis of Frizzled 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.