Description: Rabbit polyclonal antibody to PTGIS Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PTGIS. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PTGIS Alternative Names: CYP8; CYP8A1; Prostacyclin synthase; Prostaglandin I2 synthaseEntrez Gene (Human): 5740Entrez Gene (Mouse) : 19223Entrez Gene (Rat) : 25527SwissProt (Human): Q16647SwissProt (Mouse) : O35074SwissProt (Rat) : Q62969Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PTGIS expression in rat prostate (A) whole cell lysates.
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  Immunohistochemical analysis of PTGIS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.