Description: Rabbit polyclonal antibody to ARHGEF11 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ARHGEF11. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ARHGEF11 Alternative Names: KIAA0380; Rho guanine nucleotide exchange factor 11; PDZ-RhoGEFEntrez Gene (Human): 9826SwissProt (Human): O15085Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ARHGEF11 expression in Raw264.7 (A), HepG2 (B), MCF7 (C) whole cell lysates.
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Immunohistochemical analysis of ARHGEF11 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.