Description: Rabbit polyclonal antibody to RASGEF1A Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RASGEF1A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RASGEF1A Alternative Names: Ras-GEF domain-containing family member 1AEntrez Gene (Human): 221002SwissProt (Human): Q8N9B8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RASGEF1A expression in MCF7 (A), U87MG (B) whole cell lysates.
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  Immunohistochemical analysis of RASGEF1A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.