Description: Rabbit polyclonal antibody to GUF1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GUF1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GUF1 Alternative Names: Translation factor GUF1 mitochondrial; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocaseEntrez Gene (Human): 60558SwissProt (Human): Q8N442Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GUF1 expression in SHSY5Y (A) whole cell lysates.
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  Immunohistochemical analysis of GUF1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.