Description: Rabbit polyclonal antibody to ELOA3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ELOA3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: ELOA3; ELOA3B; ELOA3D Alternative Names: TCEB3B; TCEB3L; RNA polymerase II transcription factor SIII subunit A2; Elongin-A2; EloA2; Transcription elongation factor B polypeptide 3B; TCEB3C; TCEB3L2; RNA polymerase II transcription factor SIII subunit A3; Elongin-A3; EloA3; Transcription elongation factor B polypeptide 3CEntrez Gene (Human): 51224; 162699SwissProt (Human): Q8IYF1; Q8NG57; Q3SY89; A6NLF2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ELOA3 expression in SKOVCAR3 (A), Panc1 (B) whole cell lysates.
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Immunofluorescent analysis of ELOA3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.