Description: Rabbit polyclonal antibody to CDC16 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC16. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CDC16 Alternative Names: ANAPC6; Cell division cycle protein 16 homolog; Anaphase-promoting complex subunit 6; APC6; CDC16 homolog; CDC16Hs; Cyclosome subunit 6Entrez Gene (Human): 8881Entrez Gene (Mouse) : 69957SwissProt (Human): Q13042SwissProt (Mouse) : Q8R349Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDC16 expression in MCF7 (A), A549 (B) whole cell lysates.
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Immunohistochemical analysis of CDC16 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.