Description: Rabbit polyclonal antibody to CDK5RAP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDK5RAP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CDK5RAP1 Alternative Names: C20orf34; CDK5 regulatory subunit-associated protein 1; CDK5 activator-binding protein C42Entrez Gene (Human): 51654Entrez Gene (Mouse) : 66971SwissProt (Human): Q96SZ6SwissProt (Mouse) : Q8BTW8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDK5RAP1 expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of CDK5RAP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.