Description: Rabbit polyclonal antibody to FOXB1/2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXB1/2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: FOXB1; FOXB2 Alternative Names: FOXB1; FKH5; Forkhead box protein B1; Transcription factor FKH-5; FOXB2; Forkhead box protein B2Entrez Gene (Human): 27023; 442425Entrez Gene (Mouse) : 64290; 14240SwissProt (Human): Q99853; Q5VYV0SwissProt (Mouse) : Q64732; Q64733Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of FOXB1/2 expression in SG7901 (A), Myla2059 (B), mouse embryo (C), rat testis (D) whole cell lysates.
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Immunohistochemical analysis of FOXB1/2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.