Description: Rabbit polyclonal antibody to DUSP19 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DUSP19. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: DUSP19 Alternative Names: DUSP17; LMWDSP3; SKRP1; Dual specificity protein phosphatase 19; Dual specificity phosphatase TS-DSP1; Low molecular weight dual specificity phosphatase 3; LMW-DSP3; Protein phosphatase SKRP1; Stress-activated protein kinase pathway-regulating phosphatase 1; SAPK pathway-regulating phosphatase 1Entrez Gene (Human): 142679SwissProt (Human): Q8WTR2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DUSP19 expression in Panc1 (A) whole cell lysates.
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  Immunohistochemical analysis of DUSP19 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of DUSP19 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.