Description: Rabbit polyclonal antibody to Alpha-2B Adrenergic Receptor Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Alpha-2B Adrenergic Receptor. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: ADRA2B Alternative Names: ADRA2L1; ADRA2RL1; Alpha-2B adrenergic receptor; Alpha-2 adrenergic receptor subtype C2; Alpha-2B adrenoreceptor; Alpha-2B adrenoceptor; Alpha-2BAREntrez Gene (Human): 151Entrez Gene (Rat) : 24174SwissProt (Human): P18089SwissProt (Rat) : P19328Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Alpha-2B Adrenergic Receptor expression in Hela (A) whole cell lysates.
• 
  Immunohistochemical analysis of Alpha-2B Adrenergic Receptor staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
• 
  Immunofluorescent analysis of Alpha-2B Adrenergic Receptor staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.