Description: Rabbit polyclonal antibody to FARP2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FARP2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: FARP2 Alternative Names: KIAA0793; PLEKHC3; FERM RhoGEF and pleckstrin domain-containing protein 2; FERM domain including RhoGEF; FIR; Pleckstrin homology domain-containing family C member 3; PH domain-containing family C member 3Entrez Gene (Human): 9855Entrez Gene (Mouse) : 227377SwissProt (Human): O94887SwissProt (Mouse) : Q91VS8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FARP2 expression in A375 (A), LO2 (B), CT26 (C), PMVEC (D) whole cell lysates.
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  Immunohistochemical analysis of FARP2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of FARP2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.