Description: Rabbit polyclonal antibody to SKP1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SKP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SKP1 Alternative Names: EMC19; OCP2; SKP1A; TCEB1L; S-phase kinase-associated protein 1; Cyclin-A/CDK2-associated protein p19; Organ of Corti protein 2; OCP-2; Organ of Corti protein II; OCP-II; RNA polymerase II elongation factor-like protein; SIII; Transcription elongation factor B; p19A; p19skp1Entrez Gene (Human): 6500Entrez Gene (Mouse) : 21402Entrez Gene (Rat) : 287280SwissProt (Human): P63208SwissProt (Mouse) : Q9WTX5SwissProt (Rat) : Q6PEC4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SKP1 expression in CT26 (A), C6 (B), MCF7 (C), A2780 (D), Hela (E) whole cell lysates.
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  Immunohistochemical analysis of SKP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.