Description: Rabbit polyclonal antibody to p130 Cas Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p130 Cas. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: BCAR1 Alternative Names: CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130casEntrez Gene (Human): 9564Entrez Gene (Mouse) : 12927Entrez Gene (Rat) : 25414SwissProt (Human): P56945SwissProt (Mouse) : Q61140SwissProt (Rat) : Q63767Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of p130 Cas expression in Beas2B (A) whole cell lysates.
-
Immunohistochemical analysis of p130 Cas staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.