Description: Rabbit polyclonal antibody to Alpha-tubulin (Phospho-Y272) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y272 of human Alpha-tubulin protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: TUBA1A Alternative Names: TUBA1A; TUBA3; Tubulin alpha-1A chain; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain; TUBA1B; Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1C; TUBA6; Tubulin alpha-1C chain; Alpha-tubulin 6; Tubulin alpha-6 chain; TUBA3C; TUBA2; TUBA3D; Tubulin alpha-3C/D chain; Alpha-tubulin 2; Alpha-tubulin 3C/D; Tubulin alpha-2 chain; TUBA3E; Tubulin alpha-3E chain; Alpha-tubulin 3E; TUBA4A; TUBA1; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chainEntrez Gene (Human): 7846; 10376; 84790; 113457; 7278; 112714; 7277Entrez Gene (Mouse) : 22142; 22143; 22146Entrez Gene (Rat) : 64158; 500929; 100909441SwissProt (Human): Q71U36; P68363; Q9BQE3; Q13748; Q6PEY2; P68366SwissProt (Mouse) : P68369; P05213; P68373SwissProt (Rat) : P68370; Q6P9V9; Q6AYZ1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Alpha-tubulin (Phospho-Y272) expression in U87MG (A), A375 (B), Hela (C), A2780 (D), EC9706 (E), C6 (F), CT26 (G) whole cell lysates.
• 
  Immunohistochemical analysis of Alpha-tubulin (Phospho-Y272) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.