Description: Rabbit polyclonal antibody to RRN3 (Phospho-S649) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S649 of human RRN3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RRN3 Alternative Names: TIFIA; RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IAEntrez Gene (Human): 54700SwissProt (Human): Q9NYV6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of RRN3 (Phospho-S649) expression in A375 (A), EC9706 (B), SGC7901 (C) whole cell lysates.
• 
  Immunohistochemical analysis of RRN3 (Phospho-S649) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.