Description: Rabbit polyclonal antibody to MK2 (Phospho-S272) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S272 of human MK2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MAPKAPK2 Alternative Names: MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2Entrez Gene (Human): 9261Entrez Gene (Mouse) : 17164SwissProt (Human): P49137SwissProt (Mouse) : P49138Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MK2 (Phospho-S272) expression in SGC7901 (A), LO2 (B) whole cell lysates.
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  Immunohistochemical analysis of MK2 (Phospho-S272) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.