Description: Rabbit polyclonal antibody to PALF (Phospho-S116) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S116 of human PALF protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: APLF Alternative Names: C2orf13; PALF; XIP1; Aprataxin and PNK-like factor; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; XRCC1-interacting protein 1Entrez Gene (Human): 200558SwissProt (Human): Q8IW19Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PALF (Phospho-S116) expression in A2780 (A) whole cell lysates.
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Immunohistochemical analysis of PALF (Phospho-S116) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.