Description: Rabbit polyclonal antibody to HSF1 (Phospho-S121) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S121 of human HSF1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: HSF1 Alternative Names: HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1Entrez Gene (Human): 3297Entrez Gene (Mouse) : 15499SwissProt (Human): Q00613SwissProt (Mouse) : P38532Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of HSF1 (Phospho-S121) expression in mouse lung (A), rat spleen (B) whole cell lysates.
• 
  Immunohistochemical analysis of HSF1 (Phospho-S121) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.