Description: Rabbit polyclonal antibody to ASK1 (Phospho-T838) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T838 of human ASK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MAP3K5 Alternative Names: ASK1; MAPKKK5; MEKK5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5Entrez Gene (Human): 4217Entrez Gene (Mouse) : 26408SwissProt (Human): Q99683SwissProt (Mouse) : O35099Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ASK1 (Phospho-T838) expression in K562 (A) whole cell lysates.
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  Immunohistochemical analysis of ASK1 (Phospho-T838) staining in human prostate formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.