Description: Rabbit polyclonal antibody to GLUR1 (Phospho-S863) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S863 of human GLUR1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GRIA1 Alternative Names: GLUH1; GLUR1; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; Glutamate receptor ionotropic AMPA 1; GluA1Entrez Gene (Human): 2890Entrez Gene (Mouse) : 14799Entrez Gene (Rat) : 50592SwissProt (Human): P42261SwissProt (Mouse) : P23818SwissProt (Rat) : P19490Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GLUR1 (Phospho-S863) expression in HCT116 (A), U87MG (B) whole cell lysates.
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Immunohistochemical analysis of GLUR1 (Phospho-S863) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.