Description: Rabbit polyclonal antibody to NF-kappaB p100 (Phospho-S869) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S869 of human NF-kappaB p100 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/100) Gene Symbol: NFKB2 Alternative Names: LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10Entrez Gene (Human): 4791Entrez Gene (Mouse) : 18034SwissProt (Human): Q00653SwissProt (Mouse) : Q9WTK5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NF-kappaB p100 (Phospho-S869) expression in Myla2059 (A) whole cell lysates.
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  Immunohistochemical analysis of NF-kappaB p100 (Phospho-S869) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.