Description: Rabbit polyclonal antibody to GEF H1 (Phospho-S885) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S885 of human GEF H1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ARHGEF2 Alternative Names: KIAA0651; LFP40; Rho guanine nucleotide exchange factor 2; Guanine nucleotide exchange factor H1; GEF-H1; Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40Entrez Gene (Human): 9181Entrez Gene (Mouse) : 16800Entrez Gene (Rat) : 310635SwissProt (Human): Q92974SwissProt (Mouse) : Q60875SwissProt (Rat) : Q5FVC2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GEF H1 (Phospho-S885) expression in HEK293T (A), Myla2059 (B) whole cell lysates.
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  Immunohistochemical analysis of GEF H1 (Phospho-S885) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.