Description: Rabbit polyclonal antibody to RGS1/8/16 (Phospho-Y187/159/168) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y187/159/168 of human RGS1/8/16 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: RGS1; RGS8; RGS16 Alternative Names: RGSR; Regulator of G-protein signaling 16; RGS16; A28-RGS14P; Retinal-specific RGS; RGS-r; hRGS-r; Retinally abundant regulator of G-protein signalingEntrez Gene (Human): 6004Entrez Gene (Mouse) : 19734SwissProt (Human): Q08116; O15492SwissProt (Mouse) : P97428SwissProt (Rat) : P56700Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RGS1/8/16 (Phospho-Y187/159/168) expression in mouse eyes (A), rat eyes (B) whole cell lysates.
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Immunohistochemical analysis of RGS1/8/16 (Phospho-Y187/159/168) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.