Description: Rabbit polyclonal antibody to JAK3 (Phospho-Y785) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y785 of human JAK3 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: JAK3 Alternative Names: Tyrosine-protein kinase JAK3; Janus kinase 3; JAK-3; Leukocyte janus kinase; L-JAKEntrez Gene (Human): 3718Entrez Gene (Mouse) : 16453SwissProt (Human): P52333SwissProt (Mouse) : Q62137Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of JAK3 (Phospho-Y785) expression in Myla2059 (A), K562 (B), Jurkat (C) whole cell lysates.
-
Immunohistochemical analysis of JAK3 (Phospho-Y785) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.