Description: Rabbit polyclonal antibody to FOXO1/3/4 (Phospho-T24/32) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T24/32 of human FOXO1/3/4 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: FOXO1; FOXO3; FOXO4 Alternative Names: FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1Entrez Gene (Human): 2308; 4303Entrez Gene (Mouse) : 56458; 56484; 54601Entrez Gene (Rat) : 84482SwissProt (Human): Q12778; O43524; P98177SwissProt (Mouse) : Q9R1E0; Q9WVH4; Q9WVH3SwissProt (Rat) : G3V7R4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FOXO1/3/4 (Phospho-T24/32) expression in HepG2 (A), Panc1 (B), mouse lung (C) whole cell lysates.
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  Immunohistochemical analysis of FOXO1/3/4 (Phospho-T24/32) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.