Description: Rabbit polyclonal antibody to GRLF1 (Phospho-Y1105) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1105 of human GRLF1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ARHGAP35 Alternative Names: GRF1; GRLF1; KIAA1722; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-AEntrez Gene (Human): 2909Entrez Gene (Mouse) : 232906SwissProt (Human): Q9NRY4SwissProt (Mouse) : Q91YM2SwissProt (Rat) : P81128Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GRLF1 (Phospho-Y1105) expression in C6 (A), Jurkat (B), K562 (C) whole cell lysates.
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  Immunohistochemical analysis of GRLF1 (Phospho-Y1105) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.