Description: Rabbit polyclonal antibody to ABL1/2 (Phospho-Y393/439) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y393/439 of human ABL1/2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ABL1; ABL2 Alternative Names: ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150; ABL2; ABLL; ARG; Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARGEntrez Gene (Human): 25; 27Entrez Gene (Mouse) : 11350SwissProt (Human): P00519; P42684SwissProt (Mouse) : P00520; Q4JIM5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ABL1/2 (Phospho-Y393/439) expression in AML12 (A), HCT116 (B), LO2 (C) whole cell lysates.
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Immunohistochemical analysis of ABL1/2 (Phospho-Y393/439) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.