Description: Rabbit polyclonal antibody to Synaptotagmin (Phospho-S309/306) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S309/306 of human Synaptotagmin protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SYT1; SYT2 Alternative Names: SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytIIEntrez Gene (Human): 6857; 127833Entrez Gene (Mouse) : 20979; 20980Entrez Gene (Rat) : 25716; 24805SwissProt (Human): P21579; Q8N9I0SwissProt (Mouse) : P46096; P46097SwissProt (Rat) : P21707; P29101Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Synaptotagmin (Phospho-S309/306) expression in A375 (A) whole cell lysates.
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  Immunohistochemical analysis of Synaptotagmin (Phospho-S309/306) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.