Description: Rabbit polyclonal antibody to CD70 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD70. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CD70 Alternative Names: CD27L; CD27LG; TNFSF7; CD70 antigen; CD27 ligand; CD27-L; Tumor necrosis factor ligand superfamily member 7; CD70Entrez Gene (Human): 970Entrez Gene (Mouse) : 21948SwissProt (Human): P32970SwissProt (Mouse) : O55237Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD70 expression in Myla2059 (A), HCT116 (B), CT26 (C) whole cell lysates.
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Immunohistochemical analysis of CD70 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.