Description: Rabbit polyclonal antibody to CD132 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD132. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: IL2RG Alternative Names: Cytokine receptor common subunit gamma; Interleukin-2 receptor subunit gamma; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; gammaC; p64; CD132Entrez Gene (Human): 3561Entrez Gene (Mouse) : 16186SwissProt (Human): P31785SwissProt (Mouse) : P34902Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD132 expression in Myla2059 (A), HCT116 (B), mouse lung (C), rat spleen (D) whole cell lysates.
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Immunohistochemical analysis of CD132 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.