Description: Rabbit polyclonal antibody to Occludin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Occludin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: OCLN Alternative Names: OccludinEntrez Gene (Human): 100506658Entrez Gene (Mouse) : 18260Entrez Gene (Rat) : 83497SwissProt (Human): Q16625SwissProt (Mouse) : Q61146SwissProt (Rat) : Q6P6T5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Occludin expression in mouse kidney (A), rat kidney (B) whole cell lysates.
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Immunofluorescent analysis of Occludin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).