Description: Rabbit polyclonal antibody to Leptin Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Leptin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: LEP Alternative Names: OB; OBS; Leptin; Obese protein; Obesity factorEntrez Gene (Human): 3952Entrez Gene (Mouse) : 16846SwissProt (Human): P41159SwissProt (Mouse) : P41160Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Leptin expression in LO2 (A), HCT116 (B), MCF7 (C), A549 (D) whole cell lysates.
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  Immunohistochemical analysis of Leptin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.