Description: Rabbit polyclonal antibody to CXCL3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CXCL3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CXCL3 Alternative Names: GRO3; GROG; SCYB3; C-X-C motif chemokine 3; GRO-gamma(1-73); Growth-regulated protein gamma; GRO-gamma; Macrophage inflammatory protein 2-beta; MIP2-betaEntrez Gene (Human): 2921Entrez Gene (Mouse) : 330122Entrez Gene (Rat) : 171551SwissProt (Human): P19876SwissProt (Mouse) : Q6W5C0SwissProt (Rat) : Q10746Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CXCL3 expression in H9C2 (A), AML12 (B) whole cell lysates.
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Immunohistochemical analysis of CXCL3 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.