Description: Rabbit polyclonal antibody to NMDAR2D Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NMDAR2D. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GRIN2D Alternative Names: GluN2D; NMDAR2D; Glutamate receptor ionotropic NMDA 2D; GluN2D; EB11; Glutamate [NMDA] receptor subunit epsilon-4; N-methyl D-aspartate receptor subtype 2D; NMDAR2D; NR2DEntrez Gene (Human): 2906Entrez Gene (Mouse) : 14814Entrez Gene (Rat) : 24412SwissProt (Human): O15399SwissProt (Mouse) : Q03391SwissProt (Rat) : Q62645Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NMDAR2D expression in PC3 (A), A549 (B), 3T3L1 (C), PC12 (D) whole cell lysates.
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Immunohistochemical analysis of NMDAR2D staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.