Description: Rabbit polyclonal antibody to CD322 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD322. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: JAM2 Alternative Names: C21orf43; VEJAM; Junctional adhesion molecule B; JAM-B; Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; CD322Entrez Gene (Human): 58494Entrez Gene (Mouse) : 67374SwissProt (Human): P57087SwissProt (Mouse) : Q9JI59Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD322 expression in A549 (A), HEK293T (B), MEF (C) whole cell lysates.
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Immunohistochemical analysis of CD322 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.