Description: Rabbit polyclonal antibody to ULK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ULK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ULK1 Alternative Names: KIAA0722; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1Entrez Gene (Human): 8408Entrez Gene (Mouse) : 22241SwissProt (Human): O75385SwissProt (Mouse) : O70405Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ULK1 expression in A549 (A), HEK293T (B), AML12 (C), C6 (D), Panc1 (E) whole cell lysates.
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Immunohistochemical analysis of ULK1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.