Description: Rabbit polyclonal antibody to APOBEC3D Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human APOBEC3D. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: APOBEC3D; APOBEC3F Alternative Names: APOBEC3D; Probable DNA dC->dU-editing enzyme APOBEC-3D; APOBEC3F; DNA dC->dU-editing enzyme APOBEC-3F; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3FEntrez Gene (Human): 200316; 140564SwissProt (Human): Q96AK3; Q8IUX4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of APOBEC3D expression in H1792 (A), SKOVCAR3 (B), H9C2 (C), MEF (D) whole cell lysates.
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Immunohistochemical analysis of APOBEC3D staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.