Description: Rabbit polyclonal antibody to PIGY Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human PIGY. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PIGY Alternative Names: Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y; Phosphatidylinositol-glycan biosynthesis class Y protein; PIG-YEntrez Gene (Human): 84992Entrez Gene (Mouse) : 66268SwissProt (Human): Q3MUY2SwissProt (Mouse) : P0C1P0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PIGY expression in C6 (A), AML12 (B), rat kidney (C) whole cell lysates.
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  Immunohistochemical analysis of PIGY staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.