Description: Rabbit polyclonal antibody to RPL31 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RPL31. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RPL31 Alternative Names: 60S ribosomal protein L31Entrez Gene (Human): 6160Entrez Gene (Mouse) : 114641Entrez Gene (Rat) : 64298SwissProt (Human): P62899SwissProt (Mouse) : P62900SwissProt (Rat) : P62902Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RPL31 expression in A375 (A), MCF7 (B), HCT116 (C), CT26 (D), C6 (E) whole cell lysates.
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Immunohistochemical analysis of RPL31 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.