Description: Rabbit polyclonal antibody to MED21 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MED21. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MED21 Alternative Names: SRB7; SURB7; Mediator of RNA polymerase II transcription subunit 21; Mediator complex subunit 21; RNA polymerase II holoenzyme component SRB7; RNAPII complex component SRB7; hSrb7Entrez Gene (Human): 9412Entrez Gene (Mouse) : 108098SwissProt (Human): Q13503SwissProt (Mouse) : Q9CQ39Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MED21 expression in PC12 (A), CT26 (B), HEK293T (C), SGC7901 (D), HCT116 (E) whole cell lysates.
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  Immunohistochemical analysis of MED21 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.