Description: Rabbit polyclonal antibody to Von Hippel Lindau Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Von Hippel Lindau. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: VHL Alternative Names: Von Hippel-Lindau disease tumor suppressor; Protein G7; pVHLEntrez Gene (Human): 7428Entrez Gene (Mouse) : 22346Entrez Gene (Rat) : 24874SwissProt (Human): P40337SwissProt (Mouse) : P40338SwissProt (Rat) : Q64259Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Von Hippel Lindau expression in C6 (A), H1792 (B), HEK293T (C) whole cell lysates.
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Immunohistochemical analysis of Von Hippel Lindau staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.