Description: Rabbit polyclonal antibody to c-FER Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human c-FER. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: FER Alternative Names: TYK3; Tyrosine-protein kinase Fer; Feline encephalitis virus-related kinase FER; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; Proto-oncogene c-Fer; Tyrosine kinase 3; p94-FerEntrez Gene (Human): 2241Entrez Gene (Mouse) : 14158Entrez Gene (Rat) : 301737SwissProt (Human): P16591SwissProt (Mouse) : P70451SwissProt (Rat) : P09760Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of c-FER expression in mouse brain (A), rat brain (B), A2780 (C), K562 (D), H1792 (E), HCT116 (F) whole cell lysates.
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Immunohistochemical analysis of c-FER staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.